These are the current production substrate recipes being used:
Wild Bird Seed (5lbs)
5 cups Wild Bird Seed
Tablespoon Coffee
Teaspoon Gypsum
Pasteurizing water as described by Mycologyst.art
Master's Mix(5lbs)
2.5 cups hardwood pellets
2.5 cups soy hull pellets
Teaspoon Gypsum
1.4L water
Inoculating Oyster at 11% or 1:8 spawn ratio.
Inoculating Lion's Mane at 14% or 1:6 spawn ratio.
Bran 20 Substrate (5lbs)
5 cups hardwood pellets
1 cups rice bran
Teaspoon Gypsum
6 cups water
Inoculating Reishi at 9% or 1:10 spawn ratio
Inoculating Oyster at 11% or 1:8 spawn ratio.
Inoculating Lion's Mane at 14% or 1:6 spawn ratio.
AGAR (~30 plates)
1L of water
20g agar
20g malt extract
2g nutritional yeast
Liquid Culture (1500mL)
20mL Karo
4.0g malt
4.0g dextrose
2.5g peptone
1.5g rye
1.5g gypsum
1.5g brown rice flour
1.5g nutritional yeast
1500mL distilled water
Coco Coir/Vermiculite (7.5 lbs)
1 block (650 gram) coco coir
8 cups vermiculite
2 cups peat moss
.5 cups worm castings
.25 cups calcium carbonate
16 cups water
Inoculate at 25-50% or 1:2 spawn ratio
Manure/Coir/Vermiculite (15 lbs) (50%/25%/25%)
1 block (650 gram) coco coir
8 cups vermiculite
2 cups peat moss
.5 cups worm castings
.25 cups calcium carbonate
16 cups horse manure
.5 cups Gypsum
20 cups water
Inoculate at 25-50% or 1:2 spawn ratio
The following recipes are being tested/evaluated:
Pasteurized/Subsidized Hardwood (1lb)
10%
.9 cups pasteurized hardwood pellets
.1 cups soy hull pellets (attempting both pasteurized and sterilized)
Pinch gypsum
.25L water
20%
.8 cups pasteurized hardwood pellets
.2 cups soy hull pellets (attempting both pasteurized and sterilized)
Pinch gypsum
.25L water
30%
.7 cups pasteurized hardwood pellets
.3 cups soy hull pellets (attempting both pasteurized and sterilized)
Pinch gypsum
.25L water
40%
.6 cups pasteurized hardwood pellets
.4 cups soy hull pellets (attempting both pasteurized and sterilized)
Pinch gypsum
.25L water
50%
.5 cups pasteurized hardwood pellets
.5 cups soy hull pellets (attempting both pasteurized and sterilized)
Pinch gypsum
.25L water
Pasteurized/Subsidized Hardwood - 75/25 (7.5lb)
6 cups hardwood pellets
2 cups soy hull pellets
Teaspoon Gypsum
2.25L water
Inoculating Reishi at 9% or 1:10 spawn ratio
Liquid Culture - Take 1 (500mL)
8g malt
1.5g dextrose
0.5g rye
0.2g gypsum
Pinch wheat straw
Pinch millet
Pinch bird seed
Pinch nutritional yeast
Pinch potato flakes
Pinch coco coir
Pinch hardwood pellet
Pinch coffee
500mL filtered water
Liquid Culture - Take 2 (1500mL)
20mL Karo
4.0g malt
4.0g dextrose
2.5g peptone
1.5g rye
1.5g gypsum
1.5g brown rice flour
1.5g nutritional yeast
1500mL distilled water
* Removing coffee, coco coir and hardwood pellets due to their darkening the culture and making identification of contamination more difficult.
* Removing wheat straw, bird seed, coco coir, and hardwood pellets due to their insolubility
* Adding Karo and peptone while decreasing malt and dextrose concentration per MyYCO